rabbit polyclonal antiegfr d38b1 xp Search Results


anti egfr d38b1 xp rabbit mab  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc anti egfr d38b1 xp rabbit mab
Anti Egfr D38b1 Xp Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti egfr  (Cell Signaling Technology Inc)
98
Cell Signaling Technology Inc rabbit anti egfr
Rabbit Anti Egfr, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti egfr  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc anti egfr
Anti Egfr, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antiegfr rabbit monoclonal antibody d38b1  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc antiegfr rabbit monoclonal antibody d38b1
Antiegfr Rabbit Monoclonal Antibody D38b1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti egfr antibody  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc anti egfr antibody
STAP-2–derived 2D5 peptide inhibits cancer cell proliferation. A , HEK293T cells were transfected with expression vectors for the FLAG-STAP-2 PH domain <t>and</t> <t>GST-EGFR</t> cytosol domain, and then lysates were pulled down using glutathione beads at 48 h posttransfection and blotted. B – D , DU145 cells and MCF7 cells were treated with STAP-2–derived peptides at the indicated concentrations for 48 h, and then relative cell viability was measured by Cell Titer glo assay. E , DU145 cells were treated with 50 μM 2D5 peptide for 72 h. Percentage of the cells that were Annexin V and/or PI positive was analyzed by flow cytometry. F , DU145 cells were treated with 0.3 μM FITC-conjugated 2D5 and ΔR8-2D5 peptides for 30 min, and then FITC-positive DU145 cells were analyzed by flow cytometry. G , DU145 cells were treated with truncated 2D5 peptides, and then cell viability was measured as described in ( B ). H , various cell lines were treated with 2D5 peptide, and then cell viability was measured as described in ( B ). T47D cells and MDA-MB-453 cells were treated with 2D5 peptide for 96 h, and then cell viability was measured. I , DU145 cells were treated with 1, 10, 20, 30, 40, and 50 μM 2D5 peptide for 48 h, and then relative cell viability was measured by Cell Titer glo assay. n = 3. Mean values and SDs are shown. EGFR, epidermal growth factor receptor; PH, Pleckstrin homology; PI, propidium iodide.
Anti Egfr Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti-ldlr pab novus (nbp1-06709)  (Novus Biologicals)
90
Novus Biologicals rabbit anti-ldlr pab novus (nbp1-06709)
STAP-2–derived 2D5 peptide inhibits cancer cell proliferation. A , HEK293T cells were transfected with expression vectors for the FLAG-STAP-2 PH domain <t>and</t> <t>GST-EGFR</t> cytosol domain, and then lysates were pulled down using glutathione beads at 48 h posttransfection and blotted. B – D , DU145 cells and MCF7 cells were treated with STAP-2–derived peptides at the indicated concentrations for 48 h, and then relative cell viability was measured by Cell Titer glo assay. E , DU145 cells were treated with 50 μM 2D5 peptide for 72 h. Percentage of the cells that were Annexin V and/or PI positive was analyzed by flow cytometry. F , DU145 cells were treated with 0.3 μM FITC-conjugated 2D5 and ΔR8-2D5 peptides for 30 min, and then FITC-positive DU145 cells were analyzed by flow cytometry. G , DU145 cells were treated with truncated 2D5 peptides, and then cell viability was measured as described in ( B ). H , various cell lines were treated with 2D5 peptide, and then cell viability was measured as described in ( B ). T47D cells and MDA-MB-453 cells were treated with 2D5 peptide for 96 h, and then cell viability was measured. I , DU145 cells were treated with 1, 10, 20, 30, 40, and 50 μM 2D5 peptide for 48 h, and then relative cell viability was measured by Cell Titer glo assay. n = 3. Mean values and SDs are shown. EGFR, epidermal growth factor receptor; PH, Pleckstrin homology; PI, propidium iodide.
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anti egfr d38b1 rabbit mab cst  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc anti egfr d38b1 rabbit mab cst
STAP-2–derived 2D5 peptide inhibits cancer cell proliferation. A , HEK293T cells were transfected with expression vectors for the FLAG-STAP-2 PH domain <t>and</t> <t>GST-EGFR</t> cytosol domain, and then lysates were pulled down using glutathione beads at 48 h posttransfection and blotted. B – D , DU145 cells and MCF7 cells were treated with STAP-2–derived peptides at the indicated concentrations for 48 h, and then relative cell viability was measured by Cell Titer glo assay. E , DU145 cells were treated with 50 μM 2D5 peptide for 72 h. Percentage of the cells that were Annexin V and/or PI positive was analyzed by flow cytometry. F , DU145 cells were treated with 0.3 μM FITC-conjugated 2D5 and ΔR8-2D5 peptides for 30 min, and then FITC-positive DU145 cells were analyzed by flow cytometry. G , DU145 cells were treated with truncated 2D5 peptides, and then cell viability was measured as described in ( B ). H , various cell lines were treated with 2D5 peptide, and then cell viability was measured as described in ( B ). T47D cells and MDA-MB-453 cells were treated with 2D5 peptide for 96 h, and then cell viability was measured. I , DU145 cells were treated with 1, 10, 20, 30, 40, and 50 μM 2D5 peptide for 48 h, and then relative cell viability was measured by Cell Titer glo assay. n = 3. Mean values and SDs are shown. EGFR, epidermal growth factor receptor; PH, Pleckstrin homology; PI, propidium iodide.
Anti Egfr D38b1 Rabbit Mab Cst, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti egfr antibodies d38b1  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc rabbit anti egfr antibodies d38b1
STAP-2–derived 2D5 peptide inhibits cancer cell proliferation. A , HEK293T cells were transfected with expression vectors for the FLAG-STAP-2 PH domain <t>and</t> <t>GST-EGFR</t> cytosol domain, and then lysates were pulled down using glutathione beads at 48 h posttransfection and blotted. B – D , DU145 cells and MCF7 cells were treated with STAP-2–derived peptides at the indicated concentrations for 48 h, and then relative cell viability was measured by Cell Titer glo assay. E , DU145 cells were treated with 50 μM 2D5 peptide for 72 h. Percentage of the cells that were Annexin V and/or PI positive was analyzed by flow cytometry. F , DU145 cells were treated with 0.3 μM FITC-conjugated 2D5 and ΔR8-2D5 peptides for 30 min, and then FITC-positive DU145 cells were analyzed by flow cytometry. G , DU145 cells were treated with truncated 2D5 peptides, and then cell viability was measured as described in ( B ). H , various cell lines were treated with 2D5 peptide, and then cell viability was measured as described in ( B ). T47D cells and MDA-MB-453 cells were treated with 2D5 peptide for 96 h, and then cell viability was measured. I , DU145 cells were treated with 1, 10, 20, 30, 40, and 50 μM 2D5 peptide for 48 h, and then relative cell viability was measured by Cell Titer glo assay. n = 3. Mean values and SDs are shown. EGFR, epidermal growth factor receptor; PH, Pleckstrin homology; PI, propidium iodide.
Rabbit Anti Egfr Antibodies D38b1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti egfr  (Abcam)
95
Abcam anti egfr
STAP-2–derived 2D5 peptide inhibits cancer cell proliferation. A , HEK293T cells were transfected with expression vectors for the FLAG-STAP-2 PH domain <t>and</t> <t>GST-EGFR</t> cytosol domain, and then lysates were pulled down using glutathione beads at 48 h posttransfection and blotted. B – D , DU145 cells and MCF7 cells were treated with STAP-2–derived peptides at the indicated concentrations for 48 h, and then relative cell viability was measured by Cell Titer glo assay. E , DU145 cells were treated with 50 μM 2D5 peptide for 72 h. Percentage of the cells that were Annexin V and/or PI positive was analyzed by flow cytometry. F , DU145 cells were treated with 0.3 μM FITC-conjugated 2D5 and ΔR8-2D5 peptides for 30 min, and then FITC-positive DU145 cells were analyzed by flow cytometry. G , DU145 cells were treated with truncated 2D5 peptides, and then cell viability was measured as described in ( B ). H , various cell lines were treated with 2D5 peptide, and then cell viability was measured as described in ( B ). T47D cells and MDA-MB-453 cells were treated with 2D5 peptide for 96 h, and then cell viability was measured. I , DU145 cells were treated with 1, 10, 20, 30, 40, and 50 μM 2D5 peptide for 48 h, and then relative cell viability was measured by Cell Titer glo assay. n = 3. Mean values and SDs are shown. EGFR, epidermal growth factor receptor; PH, Pleckstrin homology; PI, propidium iodide.
Anti Egfr, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


STAP-2–derived 2D5 peptide inhibits cancer cell proliferation. A , HEK293T cells were transfected with expression vectors for the FLAG-STAP-2 PH domain and GST-EGFR cytosol domain, and then lysates were pulled down using glutathione beads at 48 h posttransfection and blotted. B – D , DU145 cells and MCF7 cells were treated with STAP-2–derived peptides at the indicated concentrations for 48 h, and then relative cell viability was measured by Cell Titer glo assay. E , DU145 cells were treated with 50 μM 2D5 peptide for 72 h. Percentage of the cells that were Annexin V and/or PI positive was analyzed by flow cytometry. F , DU145 cells were treated with 0.3 μM FITC-conjugated 2D5 and ΔR8-2D5 peptides for 30 min, and then FITC-positive DU145 cells were analyzed by flow cytometry. G , DU145 cells were treated with truncated 2D5 peptides, and then cell viability was measured as described in ( B ). H , various cell lines were treated with 2D5 peptide, and then cell viability was measured as described in ( B ). T47D cells and MDA-MB-453 cells were treated with 2D5 peptide for 96 h, and then cell viability was measured. I , DU145 cells were treated with 1, 10, 20, 30, 40, and 50 μM 2D5 peptide for 48 h, and then relative cell viability was measured by Cell Titer glo assay. n = 3. Mean values and SDs are shown. EGFR, epidermal growth factor receptor; PH, Pleckstrin homology; PI, propidium iodide.

Journal: The Journal of Biological Chemistry

Article Title: A peptide derived from adaptor protein STAP-2 inhibits tumor progression by downregulating epidermal growth factor receptor signaling

doi: 10.1016/j.jbc.2022.102724

Figure Lengend Snippet: STAP-2–derived 2D5 peptide inhibits cancer cell proliferation. A , HEK293T cells were transfected with expression vectors for the FLAG-STAP-2 PH domain and GST-EGFR cytosol domain, and then lysates were pulled down using glutathione beads at 48 h posttransfection and blotted. B – D , DU145 cells and MCF7 cells were treated with STAP-2–derived peptides at the indicated concentrations for 48 h, and then relative cell viability was measured by Cell Titer glo assay. E , DU145 cells were treated with 50 μM 2D5 peptide for 72 h. Percentage of the cells that were Annexin V and/or PI positive was analyzed by flow cytometry. F , DU145 cells were treated with 0.3 μM FITC-conjugated 2D5 and ΔR8-2D5 peptides for 30 min, and then FITC-positive DU145 cells were analyzed by flow cytometry. G , DU145 cells were treated with truncated 2D5 peptides, and then cell viability was measured as described in ( B ). H , various cell lines were treated with 2D5 peptide, and then cell viability was measured as described in ( B ). T47D cells and MDA-MB-453 cells were treated with 2D5 peptide for 96 h, and then cell viability was measured. I , DU145 cells were treated with 1, 10, 20, 30, 40, and 50 μM 2D5 peptide for 48 h, and then relative cell viability was measured by Cell Titer glo assay. n = 3. Mean values and SDs are shown. EGFR, epidermal growth factor receptor; PH, Pleckstrin homology; PI, propidium iodide.

Article Snippet: The following antibodies were used for immunoblotting: anti-Myc antibody (9E10, Sigma), anti-HA antibody (C29F4, Cell Signaling Technology), anti-EGFR antibody (D38B1, Cell Signaling Technology), anti-phospho-EGFR Y1068 antibody (Cell Signaling Technology), anti-GST antibody (Z-5, Santa Cruz Biotechnology), anti-STAT3 antibody (79D7, Cell Signaling Technology), anti-phospho-STAT3 antibody (GeneTex), anti-ERK antibody (K-23, Santa Cruz Biotechnology), anti-phospho-ERK antibody (Cell Signaling Technology), anti-β-actin antibody (AC-15, Santa Cruz Biotechnology), anti-p65 antibody (D14E12, Cell Signaling Technology), anti-phospho-p65 antibody (93H1, Cell Signaling Technology), and anti-phosphotyrosine antibody (4G10, GeneTex).

Techniques: Derivative Assay, Transfection, Expressing, Glo Assay, Flow Cytometry

2D5 peptide suppresses EGFR signaling. A , HEK293T cells were transfected with expression vectors for GST-STAP-2 and EGFR-HA, and then lysates were incubated with 1 or 50 μM ΔR8-2D5 peptide for 1 h. GST-STAP-2 was pulled down and blotted. B – D , DU145 cells were serum starved for 1 h and then treated with 10 μM 2D5 peptide for 30 min. The cells were stimulated with 100 ng/ml EGF for 0, 10, and 30 min and then lysates were blotted. E , DU145 cells were treated with 10 μM 2D5 peptide for 0, 30, 60, and 120 min, and then lysates were blotted. F , DU145 cells were treated with 1 or 10 μM 2D5 peptide for 30 min, and then lysates were blotted. G , shCtrl- and shEGFR-expressing DU145 cells were treated with 2D5 peptide same as ( B ) and stimulated with 100 ng/ml EGF for 15 min, then lysates were blotted. H and I , SW620 cells were treated with 2D5 peptide and 100 ng/ml EGF same as ( B ), and then lysates were blotted. J , HEK293T cells were transfected with expression vectors for Myc-STAP-2 and EGFR-HA WT or Y1068F/Y1173F (YY/FF), and then lysates were immunoprecipitated and blotted. K , DU145 cells were treated with 10 μM 2D5 peptide, and then Ccnd1 and Survivin mRNA levels were quantified by qPCR. L , DU145 cells were treated with 25 μM 2D5 peptide and 0.1 or 1 μM gefitinib for 48 h, and then cell viability was measured. M , shCtrl- and shSTAP-2-expressing DU145 cells were treated with 25 μM 2D5 peptide for 48 h, and then cell viability was measured. N , THP-1 cells were treated with 20 ng/ml PMA for 3 days. THP-1–derived macrophages were treated with 10 μM 2D5 peptide for 1 h and then stimulated with 1 μg/ml LPS for the indicated times, and then lysates were blotted. n = 3, Mean values and SDs are shown. ∗ p < 0.05, ∗∗ p < 0.01 (paired Student’s t test). EGFR, epidermal growth factor receptor; PMA, phorbol 12-myristate 13-acetate; qPCR, quantitative PCR.

Journal: The Journal of Biological Chemistry

Article Title: A peptide derived from adaptor protein STAP-2 inhibits tumor progression by downregulating epidermal growth factor receptor signaling

doi: 10.1016/j.jbc.2022.102724

Figure Lengend Snippet: 2D5 peptide suppresses EGFR signaling. A , HEK293T cells were transfected with expression vectors for GST-STAP-2 and EGFR-HA, and then lysates were incubated with 1 or 50 μM ΔR8-2D5 peptide for 1 h. GST-STAP-2 was pulled down and blotted. B – D , DU145 cells were serum starved for 1 h and then treated with 10 μM 2D5 peptide for 30 min. The cells were stimulated with 100 ng/ml EGF for 0, 10, and 30 min and then lysates were blotted. E , DU145 cells were treated with 10 μM 2D5 peptide for 0, 30, 60, and 120 min, and then lysates were blotted. F , DU145 cells were treated with 1 or 10 μM 2D5 peptide for 30 min, and then lysates were blotted. G , shCtrl- and shEGFR-expressing DU145 cells were treated with 2D5 peptide same as ( B ) and stimulated with 100 ng/ml EGF for 15 min, then lysates were blotted. H and I , SW620 cells were treated with 2D5 peptide and 100 ng/ml EGF same as ( B ), and then lysates were blotted. J , HEK293T cells were transfected with expression vectors for Myc-STAP-2 and EGFR-HA WT or Y1068F/Y1173F (YY/FF), and then lysates were immunoprecipitated and blotted. K , DU145 cells were treated with 10 μM 2D5 peptide, and then Ccnd1 and Survivin mRNA levels were quantified by qPCR. L , DU145 cells were treated with 25 μM 2D5 peptide and 0.1 or 1 μM gefitinib for 48 h, and then cell viability was measured. M , shCtrl- and shSTAP-2-expressing DU145 cells were treated with 25 μM 2D5 peptide for 48 h, and then cell viability was measured. N , THP-1 cells were treated with 20 ng/ml PMA for 3 days. THP-1–derived macrophages were treated with 10 μM 2D5 peptide for 1 h and then stimulated with 1 μg/ml LPS for the indicated times, and then lysates were blotted. n = 3, Mean values and SDs are shown. ∗ p < 0.05, ∗∗ p < 0.01 (paired Student’s t test). EGFR, epidermal growth factor receptor; PMA, phorbol 12-myristate 13-acetate; qPCR, quantitative PCR.

Article Snippet: The following antibodies were used for immunoblotting: anti-Myc antibody (9E10, Sigma), anti-HA antibody (C29F4, Cell Signaling Technology), anti-EGFR antibody (D38B1, Cell Signaling Technology), anti-phospho-EGFR Y1068 antibody (Cell Signaling Technology), anti-GST antibody (Z-5, Santa Cruz Biotechnology), anti-STAT3 antibody (79D7, Cell Signaling Technology), anti-phospho-STAT3 antibody (GeneTex), anti-ERK antibody (K-23, Santa Cruz Biotechnology), anti-phospho-ERK antibody (Cell Signaling Technology), anti-β-actin antibody (AC-15, Santa Cruz Biotechnology), anti-p65 antibody (D14E12, Cell Signaling Technology), anti-phospho-p65 antibody (93H1, Cell Signaling Technology), and anti-phosphotyrosine antibody (4G10, GeneTex).

Techniques: Transfection, Expressing, Incubation, Immunoprecipitation, Derivative Assay, Real-time Polymerase Chain Reaction

2D5 peptide decreases EGFR stabilization. A , DU145 cells were treated with 10 μM 2D5 peptide for 1 h under serum starvation and then stimulated with 100 ng/ml EGF for 20 min. The cells were fixed and stained with anti-EGFR ( green ) and anti-LAMP1 ( red ) antibodies. B , localization of EGFR and LAMP-1 was observed by confocal microscopy. We calculated the area of total EGFR and EGFR-LAMP-1 colocalization by an ImageJ software, and the percentage of EGFR-LAMP-1/total EGFR area in each 20 cells were shown. n = 20. C , DU145 cells were treated with 10 μM cycloheximide in serum-free medium for 1 h and then treated with 50 μM 2D5 peptide for 1 h together with 10 μM cycloheximide in serum-free medium. The cells were then stimulated with 100 ng/ml EGF, and lysates were blotted. D , band intensity of EGFR/Actin in ( C ) (n = 3). Mean values and SDs are shown. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (paired Student’s t test). EGFR, epidermal growth factor receptor.

Journal: The Journal of Biological Chemistry

Article Title: A peptide derived from adaptor protein STAP-2 inhibits tumor progression by downregulating epidermal growth factor receptor signaling

doi: 10.1016/j.jbc.2022.102724

Figure Lengend Snippet: 2D5 peptide decreases EGFR stabilization. A , DU145 cells were treated with 10 μM 2D5 peptide for 1 h under serum starvation and then stimulated with 100 ng/ml EGF for 20 min. The cells were fixed and stained with anti-EGFR ( green ) and anti-LAMP1 ( red ) antibodies. B , localization of EGFR and LAMP-1 was observed by confocal microscopy. We calculated the area of total EGFR and EGFR-LAMP-1 colocalization by an ImageJ software, and the percentage of EGFR-LAMP-1/total EGFR area in each 20 cells were shown. n = 20. C , DU145 cells were treated with 10 μM cycloheximide in serum-free medium for 1 h and then treated with 50 μM 2D5 peptide for 1 h together with 10 μM cycloheximide in serum-free medium. The cells were then stimulated with 100 ng/ml EGF, and lysates were blotted. D , band intensity of EGFR/Actin in ( C ) (n = 3). Mean values and SDs are shown. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (paired Student’s t test). EGFR, epidermal growth factor receptor.

Article Snippet: The following antibodies were used for immunoblotting: anti-Myc antibody (9E10, Sigma), anti-HA antibody (C29F4, Cell Signaling Technology), anti-EGFR antibody (D38B1, Cell Signaling Technology), anti-phospho-EGFR Y1068 antibody (Cell Signaling Technology), anti-GST antibody (Z-5, Santa Cruz Biotechnology), anti-STAT3 antibody (79D7, Cell Signaling Technology), anti-phospho-STAT3 antibody (GeneTex), anti-ERK antibody (K-23, Santa Cruz Biotechnology), anti-phospho-ERK antibody (Cell Signaling Technology), anti-β-actin antibody (AC-15, Santa Cruz Biotechnology), anti-p65 antibody (D14E12, Cell Signaling Technology), anti-phospho-p65 antibody (93H1, Cell Signaling Technology), and anti-phosphotyrosine antibody (4G10, GeneTex).

Techniques: Staining, Confocal Microscopy, Software